Ethylenediamine-NN′-tetra-acetate-dependent amino acid-stimulated inactivation of mouse ovarian alkaline phosphatase activity
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چکیده
منابع مشابه
The Comparision of Mouse Uterus Alkaline Phosphatase Activity at the Preimplantation Time After Ovarian Hyperstimulation and Progesterone Injection
Purpose: -Nkaline phosphatase(ALP)is a major sialoglycoprotein cell surface and intracellularof some tissues such as reproductive system. The recent investigations showed that the activity of this enzyme altered during the pre implantation and implantaion time and this alteration may correlate with endometrial receptivity. The aim of this study is to determine ALP activity of mouse uterus after...
متن کاملa comparitive study of mouse ovarian alkaline phosphatase activity in normal and psuedopregnancies
introduction: the purpose of this study was to determine alkaline phosphatase (alp) activity of ovary after ovarian induction using pregnant mare serum gonadotropin (pmsg) and human chorionic gonadotropin (hcg) during implantation periods. material and methods: a total of 240 female nmri mice aged 6-10 weeks were selected and divided into control and hyperstimulated. these mice were rendered no...
متن کاملNucleotide and amino acid sequences of human intestinal alkaline phosphatase: close homology to placental alkaline phosphatase.
A cDNA clone for human adult intestinal alkaline phosphatase (ALP) [orthophosphoric-monoester phosphohydrolase (alkaline optimum); EC 3.1.3.1] was isolated from a lambda gt11 expression library. The cDNA insert of this clone is 2513 base pairs in length and contains an open reading frame that encodes a 528-amino acid polypeptide. This deduced polypeptide contains the first 40 amino acids of hum...
متن کاملMutation of a single amino acid converts germ cell alkaline phosphatase to placental alkaline phosphatase.
Human placental and germ cell alkaline phosphatases (PLAP and GCAP, respectively), are characterized by their differential sensitivities to inhibition by L-leucine, EDTA, and heat. Yet, they differ by only 7 amino acids at positions 15, 67, 68, 84, 241, 254, and 429 within their respective 484 residues. To determine the structural basis and the amino acid(s) involved in these physicochemical di...
متن کاملThe Effect of Amino Acid Analogues on Alkaline Phosphatase Formation
The substitution of canavanine for arginine in an arginine auxotroph of Escherichia coli K-12 allowed protein synthesis to continue but inhibited the formation of the alkaline phosphatase. Sonically disrupted extracts of cells incubated with 4C-canavanine contained 4C-protein that precipitated with antibody specific to alkaline phosphatase subunits and a fraction of 14 C-active enzyme that was ...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1975
ISSN: 0264-6021
DOI: 10.1042/bj1470259